The BioSep, based on the technology of acoustic resonance, is a non-fouling / non-clogging retention system. It can be applied in continuous mode which makes the BioSep the most favorable instrument for perfusion cultures. The BioSep can be applied in both R&D (max. 1L/day), process development and on production scale (1000L/day). Screening of cells and medium optimization typically are applications in the R&D segment. Furthermore the BioSep can be seen as the first step in the DownStream Processing. The economic advantages are evident and described in literature.The technology of the BioSep also is very attractive for applications such as concentration of cell/particles and washing (e.g. beads).

Addition of fresh medium to the bioreactor, while cells are filtered from the harvest stream by the resonator chamber and returned to the bioreactor. The BioSep can be directly mounted onto the bioreactor head plate. One standard mode of operation employs, for example, a harvest pump at the exit port of the resonator chamber, and a recirculation pump for the return of separated cells that settled from the acoustic energy field within the resonator chamber. Alternatively the BioSep system can also be set up to allow for semicontinuous operation or alternative strategies of cell recirculation.